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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been observed in lots of tumors, which leads to chromosome instability, and thus may well add to tumorigenesis. This protein was discovered to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a important role in advertising mobile-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are actually described. Two extra splice variants ended up noted but comprehensive nucleotide sequence facts just isn't yet readily available.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide normally consists of the epitope acknowledged because of the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible final results are...
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This manual outlines even further achievable optimization of mobile and immunoassay parameters to make sure the best possible outcomes are...
Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining through the blocked antibody as opposed to the antibody on your own, one can see which staining is particular.
Target Information Cyclin E belongs into the really conserved cyclin relatives, whose customers are characterized by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates in the G1-S section boundary and is also degraded as here cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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